![]() ![]() ![]() ![]() Thus, hyf expression resembles that of the hyc operon. Expression was σ 54 dependent and was inhibited by HycA, the negative transcriptional regulator of the formate regulon. This work revealed that hyf is induced under fermentative conditions by formate at a low pH and in an FhlA-dependent fashion. In order to gain insight into the physiological role of the Hyf system, we investigated hyf expression by using a hyfA-lacZ transcriptional fusion. The hyf locus ( hyfABCDEFGHIJ-hyfR-focB) of Escherichia coli encodes a putative 10-subunit hydrogenase complex (hydrogenase-4 ) a potential σ 54-dependent transcriptional activator, HyfR (related to FhlA) and a putative formate transporter, FocB (related to FocA). ![]()
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